Vadim Frolov

Ikerbasque Research Professor

Vadim Frolov earned his MS in applied physics and mathematics in 1993, from the Moscow Institute of Physics and Technology, and his PhD degree in biophysics in 1997, from Moscow State University.

Subsequently (1998-2004) he worked as researcher, senior researcher and group leader in A.N. Frumkin Institute of Electrochemistry, Moscow, followed by senior research fellow position at the National Institutes of Health, Bethesda, USA (2005-2008) and Ikerbasque research professor position at the Biophysics Unit (CSIC, UPV/EHU), Leioa, Spain (2009-present). His research is focused on nanomechanics of cellular and biomimetic membrane systems, mechanisms of membrane remodeling by proteins and development of novel experimental approaches for studying cellular membranes at nanoscale. Recent publications of his group include:

1. Stochastic transport through carbon nanotubes in lipid bilayers and live cell membranes. Geng J, Kim K, Zhang J, Escalada A, Tunuguntla R, Comolli LR, Allen FI, Shnyrova AV, Cho KR, Munoz D, Wang YM, Grigoropoulos CP, Ajo-Franklin CM, Frolov VA, Noy A. Nature (2014) 514:612-5.

2. Geometric catalysis of membrane fission driven by flexible dynamin rings. Shnyrova AV, Bashkirov PV, Akimov SA, Pucadyil TJ, Zimmerberg J, Schmid SL, Frolov VA. Science (2013) 339:1433-6.

Publications

Reconstitution and real-time quantification of membrane remodeling by single proteins and protein complexes

Bashkirov PV, Kuzmin PI, Chekashkina K, Arrasate P, Vera Lillo J, Shnyrova AV, Frolov VA.

Nat Protoc. 15(8):2443-2469. (2020) doi: 10.1038/s41596-020-0337-1. (2020)
Electrophysiological Methods for Detection of Membrane Leakage and Hemifission by Dynamin 1

Bashkirov PV, Chekashkina KV, Shnyrova AV, Frolov VA.

Methods Mol Biol. 2159:141-162 (2020). doi: 10.1007/978-1-0716-0676-6_11. (2020)
Dynamic constriction and fission of endoplasmic reticulum membranes by reticulon

Espadas J, Pendin D, Bocanegra R, Escalada A, Misticoni G, Trevisan T, Velasco Del Olmo A, Montagna A, Bova S, Ibarra B, Kuzmin PI, Bashkirov PV, Shnyrova AV, Frolov VA, Daga A.

Nat Commun. 10(1):5327 (2019). doi: 10.1038/s41467-019-13327-7. (2019)
The 2018 biomembrane curvature and remodeling roadmap

Bassereau P, Jin R,. Baumgart T, Deserno M , Dimova R, Frolov VA, Bashkirov PV, Grubmuller H, Jahn R, Risselada HJ, Johannes L, Kozlov MM, Lipowsky R, Pucadyil TJ, Zeno WF, Stachowiak JC, Stamou D, Breuer A, Lauritsen L, Simon C, Voth GA, Weikl TR.

Journal Of Physics D-Applied Physics 51, 34, 343001. doi: 10.1088/1361-6463/aacb98 (2018)
Membrane fission by dynamin: what we know and what we need to know

B. Antonny, C. Burd, P. De Camilli, E. Chen, O. Daumke, K. Faelber, M. Ford, V.A. Frolov, A. Frost, J.E. Hinshaw, T. Kirchhausen, M.M. Kozlov, M. Lenz, H.H. Low, H. McMahon, C. Merrifield, T.D. Pollard, P.J. Robinson, A. Roux and S. Schmid

EMBO J. 35, 2270-2284 (2016)
Synthesis, lipid membrane incorporation, and ion permeability testing of carbon nanotube porins

R.H. Tunuguntla, A. Escalada, V. Frolov and A. Noy

Nat Protoc. 11, 2029-2047 (2016)
Dynamin-catalyzed membrane fission occurs in two mechanistically distinct stages

Juha-Pekka Mattila*, Anna V. Shnyrova*, Anna S Sundborger, Eva Rodriguez Hortelano, Mark Fuhrmans, Sylvia Neumann, Marcus Müller, Jenny E Hinshaw, Sandra L Schmid, Vadim A Frolov , (* These authors contributed equally) ,

Nature 524, pp. 109-113. (2015)
Geometry of membrane fission

V.A. Frolov, A. Escalada, S.A. Akimov and A. Shnyrova.

Chem Phys Lipids. 185, 129-140 (2015)
Stochastic transport through carbon nanotubes in lipid bilayers and live cell membranes.

J. Geng, K. Kim, J. Zhang, A. Escalada, R. Tunuguntla, L.R. Comolli, F.I. Allen, A.V. Shnyrova, K.R. Cho, D. Muñoz, Y.M. Wang, C. P. Grigoropoulos, C.M. Ajo-Franklin, V.A. Frolov and A. Noy.

Nature 514, 612-615 (2014).
Geometric catalysis of membrane fission driven by flexible dynamin rings.

A.V. Shnyrova, P.V. Bashkirov, S.A. Akimov, T.J. Pucadyil, J. Zimmerberg, S.L. Schmid and V.A. Frolov.

Science 339, 1433-1436 (2013).
Dynamin: functional design of a membrane fission catalyst.

S.L. Schmid and V.A. Frolov.

Annu. Rev. Cell Dev. Biol. 27, 79–105 (2011).
Lipid polymorphisms and membrane shape.

Vadim A. Frolov, Anna V. Shnyrova, Joshua Zimmerberg

Cold Spring Harb Perspect Biol ;3:a004747 (2011).
Reconstitution of proapoptotic BAK function in liposomes reveals a dual role for mitochondrial lipids in the BAK-driven membrane permeabilization process.

O. Landeta, A. Landajuela, D. Gil, S. Taneva, C. DiPrimo, B. Sot, M. Valle, V.A. Frolov, and G. Basañez.

J. Biol. Chem. 286, 8213-8230 (2011).