Single-molecule Biophysics

Single-molecule Biophysics

We explore the behaviour of single-protein molecules as they are forced to translocate through nanometer-size pores inserted in lipid membranes. Measuring the ionic current through the pore, we are able to follow their unfolding and refolding pathways. Such pathways are vectorial, as those found in vivo during protein synthesis, degradation and translocation through some membrane translocons. Our aim is both to explain protein folding and unfolding in vivo, and to provide biotechnological tools for the analysis of proteins with nanopores.

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