BIOPHYSICS CONGRESS 2015

Membrane Protein Folding and Stability. Structural Motif Engineering and Design

Membrane Protein Folding and Stability. Structural Motif Engineering and Design

Protein folding has been the subject of intensive research. Well-designed combinations of experimental and computational studies are enabling folding to be followed at atomic resolution, with the result that general rules are emerging. This insight, however, pertains to water-soluble proteins and it is unclear how the unifying mechanisms extend to the many proteins that reside in membranes. Understanding membrane protein folding in vitro will not only begin to overcome the problems of overexpression, purification and solubilization of membrane proteins, but also bring new techniques to membrane protein research. We have chosen the pore-forming fragment of colicin A as a model to study protein-lipid complex formation and stability. We investigate the kinetics and thermodynamics of folding aiming to obtain mechanistic detail, with an emphasis on α-helical proteins.

Team

Senior Researchers

Collaborators

Relevant Publications